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What probe should I use?
Counting cells with Optical Fractionator
SRS image acquisition for fluorescent tissue
Measuring length with Spaceballs
ROI area/volume with Cavalieri
Area/volume in heterogeneous tissue with Area Fraction Fractionator
Cell area/volume with Nucleator
Current user guide
View detailed instructions and documentation
Older versions
v. 2018
v. 11
v.10
v.9
Other editions
SI Cleared Tissue
SI Whole Slide
Quick guides
Expand your skills
PDFs
Probes at a glance
Stereological formulas
Optical Fractionator workflow
Spaceballs workflow
Cavalieri probe
System calibration
Slide scanning
Mouse/keyboard
Accounting for missing sections
How-to videos
System calibration 1: Lens calibration
System calibration 2: Parcentric/parfocal calibration
System calibration 3: Accuracy
Visualize in 3D
Create mp4 movies
Cursor size & line thickness